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--- afm [2025/07/23 17:59] – ethanminot
+++ afm [2025/10/07 12:02] (current) – [Step-by-step walk through for AC mode imaging] ethanminot
@@ Line 14: / Line 14: @@
 Basic rules about booking time:
   *People who have scheduled time get priority.
-  *Don't block off an entire 9am-5pm workday - leave at least an hour for someone to do a quick characterization.
+  *For OSU internal users, please limit your bookings to 3 hours per day. On the day of your booking, you can extend your time if no one else has booked time.
+  *Only make a booking if you are 90% sure you will use the booking. When you make a booking, everyone else adjusts their schedule to work around your booking. Be respectful of the effort other users are making for you.
 Every time you use the AFM, follow these steps:
@@ Line 62: / Line 63: @@
 ===== Step-by-step walk through for AC mode imaging =====
-//If a tip change is needed - see the [[AFM tip change information]]//
+//If a tip change is needed - see the //
-  - Sign into the log book (black ring binder) on the table next to the AFM.
+  - Sign into the log book (black ring binder) on the table next to the AFM. If Jesse from Inpria used the AFM last, you'll need to mount the standard AFM tip back in the machine: [[AFM tip change information]]
   - Start your RELMS reservation on the [[https://relms.oregonstate.edu/facilities|RELMS website]].
   - Turn on the laser - Key switch on the AFM computer
@@ Line 81: / Line 82: @@
     * Move laser toward the tip of the cantilever - the control wheels are labeled LDX and LDY (laser deflection x and y). They are located on the back & right side of the MFP-3D tripod. Use these controls to maximize the 'Sum' signal. If the laser is badly misaligned, it can be hard to locate. If you "loss" the laser spot, ask an experienced user for help.
     * Adjust the photodetector (PD) position. The control wheel for the photodetector is located on the left side of the MFP-3D tripod and is labeled PD. Set the PD 'Deflection' meter to near zero.
-  - X Set AC Mode - In main tab of the master panel select 'AC Mode' in the 'Imaging Mode' pull down menu
   - Tune the AFM
     *  Open 'Tune' tab in the master panel
-    *  Set 'Target %' to -5.0 % (this setting favors repulsive mode imaging, often recommended for beginners)
+    *  Set 'Target %' to -5.0 %. This setting is a first guess at the ideal drive frequency (setting it slightly less than the resonant frequency). You may have to test different drive frequencies later if you notice "phase jumping" during imaging.
-    * Click the 'Auto Tune' button and wait for tuning to finish. Software will set drive frequency.
+    * Click the 'Auto Tune' button and wait for tuning to finish. Software will set drive frequency and drive amplitude. The graph of amplitude and phase should look like the textbook curves for a driven damped harmonic oscillator.
+    * In the read-out window of the software, you will now see a 1-volt amplitude signal underneath the sum signal. Maximize this amplitude signal by moving the laser along the length of the AFM cantilever. When the amplitude is maximized, the sum signal will be slightly less than its max value.
+    * Click 'Auto Tune' one more time (or manually adjust the drive amplitude).
   - Engage the tip
     * Set the I gain to 10
-    * Make the 'Set Point Voltage' about 95% of the measured amplitude signal. (The measured amplitude signal is probably 1 V right now)
+    * Make the 'Set Point Voltage' about 95% of the measured free-air amplitude signal. (The measured free-air amplitude signal is probably 1 V right now, but you need to watch this, it might be drifting.)
-    * Click 'engage' in the S&D meter and
+    * Click 'engage' in the S&D meter panel.
     * Lower the tip down towards the sample by shortening the front leg of the MFP-3D tripod. Watch the measured amplitude as you do this. Also watch the camera image. As the surface comes into focus (before the amplitude starts to drop), consider moving to a clean place on your sample (use the x-y stage coarse positioning micrometers).
-    * If you have a clean landing area for your tip, continue lowering the tip down towards the sample. The amplitude will drop as you near the surface. You will also notice changes in phase. Pay attention to these "tell tails". The computer will beep when feedback kicks in to stop the amplitude from dropping below the setpoint. Continue lowering until the Z voltage is just past the middle of its range. (If the Z-voltage is not behaving as expected, click withdraw and check the amplitude and phase).
+    * If you have a clean landing area for your tip, continue lowering the tip down towards the sample. The amplitude will drop as you near the surface. You will also notice changes in phase. Pay attention to these "tell-tales". The computer will beep when feedback kicks in to stop the amplitude from dropping below the setpoint. Continue lowering until the Z voltage is just past the middle of its range. (If the Z-voltage is not behaving as expected, click withdraw and check the amplitude and phase).
     * Lower the 'Set Point Voltage' click by click. The Z-voltage will increase.
     * When the Z-voltage is above its midpoint, use the thumb wheel to lower the tripod leg until the Z-voltage is below the midpoint.
-    * Lower the 'Set Point Voltage' again, reducing it click by click. The Z-voltage will increase. Watch for a hard stop. If the Z-voltage increases past its midpoint without a hard stop, repeat the cycle of (a) thumb wheel and (b) set point voltage.
+    * Lower the 'Set Point Voltage' again, reducing it click by click. The Z-voltage will increase. Watch for a hard stop. If the Z-voltage increases past its midpoint without a hard stop, repeat the cycle of turning the thumb wheel and lowering the set-point voltage.
     * After you achieve a hard stop, disengage the tip by clicking 'withdraw' in the S&D meter
   - Close AFM Hood gentle (don't bump the machine).
   - Check the Amplitude and Phase of the cantilever vibration. Re-tune if needed.
-  - Set image details in the main tab
+  - Set imaging parameters in the main tab. For example, you might start with a scan size of 2 microns. See the imaging rules of thumb listed below.
-  - Scan the sample. Clicking 'frame up' or 'frame down' will start a scan
+  - Do your first test scan the sample. Clicking 'frame up' or 'frame down' will start a scan. You should do a test scan before you spend much time searching for a specific location on your sample.
+  - Observe the quality of the scan. See the section below called "Tuning the AFM to get a good image". Look for phase jumps. Look for parachuting. Look for the tip loosing contact with the surface. Remedies are described below.
+  - If you got a good image, you are ready for the rest of your session. If you need the microscope to search a large area, raise the tip by one "swipe" of the thumbwheel so you can safely make coarse motion in the x-y plane.
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   - Click 'Stop!!!' button to stop the current scan and withdraw the tip
   - Open AFM Hood
-  - Manually retract tip from sample - Give the front thumbwheel a few clockwise twists
+  - Manually retract tip from sample - turn the front thumbwheel in the "up" direction. Watch the camera feed and the tip amplitude to ensure you aren't inadvertently crashing the tip into the sample.
-  - Turn off laser - Key on the AFM computer
+  - Turn off laser - Key on the AFM controller.
-  - Turn off camera light - Switch on the box sitting on top of the AFM
+  - Turn off camera light - Switch on the box sitting on top of the AFM enclosure.
-  - Place MFP-3D onto its shelf holder
+  - Place MFP-3D tripod onto its shelf holder
   - Remove sample
-  - Close software and log out
+  - Close the AFM Hood to keep dust out of the machine.
+  - Close software.
+  - Sign out of the Log Book.
+  - End the reservation in RELMS.
   - Leave controller and PC running unless expecting a power outage
-===== Imaging rules of thumb =====
+===== Tuning the AFM to get a good image =====
+Check the **scan rate**. Is it less than 15 microns/second? Slower scans are more stable and reproducible. You might save time in the long run (and headache) by keeping scan rate less than 15 microns/second. Use an even slower scan rate if the features on the sample are very tall.
+When you withdraw from the surface, check the free-air amplitude is the same as you when you first tuned the tip. If **free-air amplitude has drifted**, you can manually change the drive amplitude.
+Sometimes the image is improved by withdrawing and re-running the autotune procedure (**resonant frequency might have changed**). The autotune procedure will make changes to both the drive amplitude and drive frequency.
+Sometimes the image is improved by lowering the set-point amplitude a few clicks. For example, this might fix **parachuting**. Minor changes to set-point amplitude can be made in real time, during imaging. The set-point should be low enough that the tip stays in contact with the surface. However, don't make the set-point too low. The ideal set-point amplitude is typically about two clicks lower than loosing contact with the surface.
+If you notice **phase jumping** (a jump from below 90 degree to above 90 degrees), you need to make adjustments to the drive frequency. Phase jumping will wear out the AFM tip (making it blunt). Phase jumping also introduces artifacts in the height data. Make test images with different values of drive frequency such that the free-air phase is 70 degrees, 80 degrees, 100 degree and 110 degrees. To make these test images, you'll need to maintain a constant free-air amplitude by simultaneously adjusting drive amplitude. By doing this, you are searching for imaging parameters for which the cantilever oscillations are most stable.
+It’s hard to predict a priori whether the best images will be acquired with phase below 90, or above 90. The best imaging regime for a given day depends on tip sharpness, cantilever stiffness, the sample’s mechanical/adhesive properties, the material and coating of the tip, and the humidity in the room. These factors modify the functional form of long-range van der Waals forces, the electrostatic forces, the way the tip indents the sample, and the capillary forces related to the water meniscus. A nonlinear tip–sample force with respect of tip-sample separation leads to bistabilities, and these bistabilities cause the phase jumps which mess up the AFM image.
+===== Other imaging rules of thumb =====
 It is easiest to get a good image on a small scan area (~ 2 micron). A small scan also facilitates verification of tip sharpness. Starting from the default settings you can fine tune the image and then start increasing the scan size. Good settings will minimize ringing and reduce shadows while keeping the scan rate reasonably fast.
 **Beginner settings**
-  *Scan size 2 micron (look at a random small feature on a flat background to verify the sharpness of the tip)
+  *Scan size 4 micron (look at a random small feature on a flat background to verify the sharpness of the tip)
   *Scan rate < 15 micron/s
   *Integral gain 10
@@ Line 123: / Line 144: @@
   *Set-point amplitude 0.75 V
-Sometimes the image is improved by lowering the set-point amplitude a few clicks. For example, this might fix parachuting.
-Sometimes the image is improved by withdrawing and re-running the autotune procedure (the resonant frequency might have changed).
-When you withdraw from the surface, check the free air amplitude is the same as you when you first tuned the tip.
-Another thing to try is a slower scan rate. The price you pay is scan time. However, I've found that you actually save time (and headache) by taking a single high quality slow scan rather than a bunch of quick ones with little parameter adjustments in between. I find that adjusting the rate so that the scan speed is <10 micron/sec works well in nearly all cases.
 **Using nanotubes as a diagnostic tool**
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   - If something is stuck to your AFM tip, it will show up in a Force curve.
-**To replace a tip**
-  - Invert the AFM head onto the stabilization table. Remove the tip holder by pressing the black rubber button and secure it in the temporary mount by the laser.
-  - Gently unscrew the clamp holding the tip down with a Phillips screwdriver. You don't want to completely unscrew it, just loosen it enough to remove the tip.
-  - Grab the old tip by its sides with a pair of tweezers and pull it away from the clamp. It should slide out freely.
-  - Remove a new cantilever from the box with a pair of plastic tweezers using a twisting motion - be careful not to touch the end with the tip! Grab the new cantilever with your tweezers so the end you want to insert into the tip holder is pointing away from you and the tip is pointing upwards.
-  - Slide the tip between the tip holder and the clamp (probably just as the old one was placed). The cantilever should be placed so it isn't inserted too far or too short. Too far, and the tip will not be well situated in the holder and zeroing the deflection will be impossible (see the figure below).
-  - Once the tip is inserted properly, tighten the clamp and replace the holder in the AFM head. Make sure to rotate the AFM head in the opposite direction you used to invert the head, to avoid twisting the head cable.
-  - Test the tip insertion two ways: i) Make sure the laser spot can be placed on the tip and the deflection can be zeroed without placing the wheels at their maximum range. ii) Auto tune the AFM, and make sure the amplitude and phase plots are free of any irregularities.
-{{AFMCantelieverPosition.png|}}
 ===== Trouble shooting =====
 AFM doesn't do what you want it to do (e.g. laser doesn't turn on or z-voltage behaves strange) it is always a good idea to